HTRF
HTRF® (Homogeneous Time Resolved Fluorescence) is a no-wash technology that combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence.
This technology can be utilized in cellular or biochemical assays, employing competitive and non-competitive formats. It may be used for drug discovery, biotherapy and basic research in therapeutic areas such as oncology, CNS and metabolic and immune disorders. Researchers can explore a wide range of applications, including GPCRs, kinases, epigenetics, biotherapy, and quantification of a range of biomarkers and cytokines.
- Easy to perform: homogeneous assay with no wash requirement
- High level of sensitivity, signal stable in time
- Time and labor savings, rapid and accurate results
- Amenable to miniaturization
- Ability to detect and analyze small and large complexes with low to high affinity interactions
- Field of applications: basic research, target discovery and validation, HTS and primary screening, secondary screening, lead optimization
For research use only. Not for use in diagnostic procedures.
Cell signaling
The phosphorylation reaction is a post-translational modification, mediated by kinases that controls the activity of proteins involved in signal transduction. It is an appropriate readout of the activation status of a pathway of interest.
The phosphorylation reaction is a post-translational modification, mediated by kinases that controls the activity of proteins involved in signal transduction. It is an appropriate readout of the activation status of a pathway of interest.
The HTRF cell signaling kits are designed to directly measure proteins within cells. We offer more than 200 ready-to-use HTRF kits for detecting endogeneous and basal levels of phosphorylated proteins. All kits are built on the same precise format and protocol.
- Cell-based assays, measure your phospho protein of interest
- No-wash, no radioactivity, no gel
- 16 μL sample
- Equal or more sensitive than Western Blot
- Pharmacological studies of pathway inhibitors, agonists, and allosteric modulators
- Compatible with cell lysates, tissue extracts, xenografts, iPS cells, 3-D cell models
GPCR research
Revvity is a leader in G protein-coupled receptor research and drug discovery, with over 100 products and compelling scientific content in many categories of GPCR Binding and signaling. Each year, hundreds of GPCR researchers around the globe trust our products to go further and faster in their research projects.
Revvity is a leader in G protein-coupled receptor research and drug discovery, with over 100 products and compelling scientific content in many categories of GPCR Binding and signaling. Each year, hundreds of GPCR researchers around the globe trust our products to go further and faster in their research projects.
- Gs-, Gi/o-, Gq-coupled receptor activation
- Agonist, antagonist, inverse agonist, and allosteric modulator screening
- Detection of slow acting agonists and antagonists
- Phosphodiesterase (PDE) monitoring
- Adenylate cyclase activity assessment
Biomarkers
HTRF kits are developed to measure released and circulating biomarkers. This offering covers multiple research areas, such as CNS, cardiovascular, inflammation, oncology, pain, metabolism, NASH, and diabetes.
HTRF kits are developed to measure released and circulating biomarkers. This offering covers multiple research areas, such as CNS, cardiovascular, inflammation, oncology, pain, metabolism, NASH, and diabetes.
- Simple add-and-read method, alternative to ELISA
- < 1 hour bench time
- Compatible with Revvity 96-well low volume plate
- All components included in Kit
Cytokines
Our HTRF kits cover the full range of cytokines: interleukins, interferons (IFN), and chemokines. All our assays enable the measurement of cytokine release in cell supernatant.
Our HTRF kits cover the full range of cytokines: interleukins, interferons (IFN), and chemokines. All our assays enable the measurement of cytokine release in cell supernatant.
Our assays have been validated in several cell lines, primary cells, and PBMCs. The low sample requirement allows the quantification of multiple cytokines in parallel, from the same supernatant.
- 16 μL sample
- < 2 hrs bench time
- Validated on PBMC
- Compatible with Revvity 96- and 384-well low-volume plates
Protein-protein interactions
We offer an extensive line of ready-to-use protein-protein interaction reagents labeled with HTRF fluorescent dyes (europium- and terbium-cryptates, XL665, and d2) for the development of novel or customer-specific assays.
We offer an extensive line of ready-to-use protein-protein interaction reagents labeled with HTRF fluorescent dyes (europium- and terbium-cryptates, XL665, and d2) for the development of novel or customer-specific assays.
A wide range of assays have already been produced with these reagents and have proven relevant when addressing interactions of widely varying sizes, affinities, and formats.
- Biotherapeutics screening
- Protein-protein interactions: small to large complexes, low to high affinity
- Nuclear receptor assays
- Protease and other enzyme assays
- Protein-DNA interaction
Epigenetics
We provides a way to explore epigenetic enzymes and proteins such as histone methyltransferases (HMT), demethylases (HDMT), deacetylases (HDAC), sirtuins, and Binding domains as druggable targets.
We provides a way to explore epigenetic enzymes and proteins such as histone methyltransferases (HMT), demethylases (HDMT), deacetylases (HDAC), sirtuins, and Binding domains as druggable targets.
We offer EPIgeneous® products, a dedicated line of Kits in biochemical and cellular formats for investigations of DNA methyltransferases (DNMTs), HDMTs, HMTs, HDACs, and Ubiquitin ligases.
- Detecting peptide, nucleosome, full histone, DNA, RNA, and small molecule modifications
- Screening and characterization of enzyme inhibitors
Kinase
Benefit from our extensive HTRF-based line of biochemical and kinase assays and reagents for analyzing serine/threonine, tyrosine kinases. This platform is available in semi-universal and kinase-specific assay formats.
Benefit from our extensive HTRF-based line of biochemical and kinase assays and reagents for analyzing serine/threonine, tyrosine kinases. This platform is available in semi-universal and kinase-specific assay formats.
All HTRF biochemical kinase assays use nonradioactive, homogeneous protocols, and are compatible with a range of ATP concentrations and substrate types, including whole proteins. We developed a line of kinase binding assays to specifically monitor kinase/inhibitor interactions and complement its existing kinase activity assay solutions. The assays can be easily miniaturized for increased throughput, reduced reagent usage, and lower enzyme consumption.
- Validated on over 200 kinases
- Low enzyme consumption
- Broad range of ATP (up to mM)
- Study of inactive or mutated kinases
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